(04-22-2012, 07:21 PM)Live-Free-Or-Pie link Wrote: Saw the Phoenix Wright movie this weekend in DC. Two word review:

FUCK. YES.

Best vidjagame adaptation ever. Saw it again next day.

(04-24-2012, 11:36 AM)Saxxy link Wrote: YAY YAY YAY my PI has officially accepted me into his lab!  I had been hella stressing about it because he was being so PC/fair about it (4 other people are also trying to get into the lab, and he's weirdly nice for a prof so he doesn't want to hurt people's feelings/crush their dreams etc.), so now maybe I can sleep a bit better.

oh who am i kidding i'm just gonna stress about finals now

but yay!

(04-24-2012, 11:36 AM)Saxxy link Wrote: YAY YAY YAY my PI has officially accepted me into his lab!  I had been hella stressing about it because he was being so PC/fair about it (4 other people are also trying to get into the lab, and he's weirdly nice for a prof so he doesn't want to hurt people's feelings/crush their dreams etc.), so now maybe I can sleep a bit better.

oh who am i kidding i'm just gonna stress about finals now

but yay!

the pay and stress suck, but I'm doing something I love and to a large extent i'm my own boss aside from what the actual boss-man wants.  Tradeoffs, yo

(04-24-2012, 04:32 PM)Saxxy link Wrote: Research: I'm joining a microbial evolution lab, my PI was previously worked with the E. coli Long-term Experimental Evolution project, so we do a lot of stuff like "hey, can you evolve a microbe to incorporate unnatural amino acids into it's proteins?" or "can a microbe evolve to be a better evolver?"

I don't have a solid, this-is-exactly-what-i'll-do project yet, but the broad outline is going to be what i'm calling "genomic remixing".  There are a lot of systems out there in nature and in labs that can transfer genes between microbes.  Generally this happens at a pretty low frequency, but there's a species of marine microbes that produce these virus-like particles called "gene transfer agents", which could potentially be used to shift relatively large amounts of DNA from a host strain to a recipient strain.  It depends on how these viral-like particles actually interact with the cell they're "infecting", but it has been shown that these guys can infect species that are like, way the hell not related to them (its kinda like the animal equivalent of a jellyfish transfering genes to a human).

Also, I'll be investigating a couple genomic systems to select for microbes that 1) lose large chunks of their genomes (whole or multiple genes at a time), or 2) duplicate chunks of their genome.

And whatever else I have time for/money/energy for, I guess.

Funding: Yeah, it depends on how much funding my PI gets, but I'm paid to be in the lab doing experiments, writing papers, etc.  We're required to do at least one semester of TAing, and it can be more depending on how our funds are.  But yeah, I'm making that sweet sweet grad school cash the pay and stress suck, but I'm doing something I love and to a large extent i'm my own boss aside from what the actual boss-man wants.  Tradeoffs, yo

let's see how happy i am next year, when I'm taking my prelims!

(04-24-2012, 07:29 PM)Didzo link Wrote: [quote author=Saxxy link=topic=3603.msg243645#msg243645 date=1335303160]
Research: I'm joining a microbial evolution lab, my PI was previously worked with the E. coli Long-term Experimental Evolution project, so we do a lot of stuff like "hey, can you evolve a microbe to incorporate unnatural amino acids into it's proteins?" or "can a microbe evolve to be a better evolver?"

I don't have a solid, this-is-exactly-what-i'll-do project yet, but the broad outline is going to be what i'm calling "genomic remixing".  There are a lot of systems out there in nature and in labs that can transfer genes between microbes.  Generally this happens at a pretty low frequency, but there's a species of marine microbes that produce these virus-like particles called "gene transfer agents", which could potentially be used to shift relatively large amounts of DNA from a host strain to a recipient strain.  It depends on how these viral-like particles actually interact with the cell they're "infecting", but it has been shown that these guys can infect species that are like, way the hell not related to them (its kinda like the animal equivalent of a jellyfish transfering genes to a human).

Also, I'll be investigating a couple genomic systems to select for microbes that 1) lose large chunks of their genomes (whole or multiple genes at a time), or 2) duplicate chunks of their genome.

And whatever else I have time for/money/energy for, I guess.

Funding: Yeah, it depends on how much funding my PI gets, but I'm paid to be in the lab doing experiments, writing papers, etc.  We're required to do at least one semester of TAing, and it can be more depending on how our funds are.  But yeah, I'm making that sweet sweet grad school cash the pay and stress suck, but I'm doing something I love and to a large extent i'm my own boss aside from what the actual boss-man wants.  Tradeoffs, yo

let's see how happy i am next year, when I'm taking my prelims!

(04-24-2012, 09:22 PM)versus link Wrote: [quote author=Didzo link=topic=3603.msg243678#msg243678 date=1335313773]
[quote author=Saxxy link=topic=3603.msg243645#msg243645 date=1335303160]
Research: I'm joining a microbial evolution lab, my PI was previously worked with the E. coli Long-term Experimental Evolution project, so we do a lot of stuff like "hey, can you evolve a microbe to incorporate unnatural amino acids into it's proteins?" or "can a microbe evolve to be a better evolver?"

I don't have a solid, this-is-exactly-what-i'll-do project yet, but the broad outline is going to be what i'm calling "genomic remixing".  There are a lot of systems out there in nature and in labs that can transfer genes between microbes.  Generally this happens at a pretty low frequency, but there's a species of marine microbes that produce these virus-like particles called "gene transfer agents", which could potentially be used to shift relatively large amounts of DNA from a host strain to a recipient strain.  It depends on how these viral-like particles actually interact with the cell they're "infecting", but it has been shown that these guys can infect species that are like, way the hell not related to them (its kinda like the animal equivalent of a jellyfish transfering genes to a human).

Also, I'll be investigating a couple genomic systems to select for microbes that 1) lose large chunks of their genomes (whole or multiple genes at a time), or 2) duplicate chunks of their genome.

And whatever else I have time for/money/energy for, I guess.

Funding: Yeah, it depends on how much funding my PI gets, but I'm paid to be in the lab doing experiments, writing papers, etc.  We're required to do at least one semester of TAing, and it can be more depending on how our funds are.  But yeah, I'm making that sweet sweet grad school cash the pay and stress suck, but I'm doing something I love and to a large extent i'm my own boss aside from what the actual boss-man wants.  Tradeoffs, yo

let's see how happy i am next year, when I'm taking my prelims!

(04-24-2012, 10:46 PM)Saxxy link Wrote: [quote author=versus link=topic=3603.msg243685#msg243685 date=1335320548]
[quote author=Didzo link=topic=3603.msg243678#msg243678 date=1335313773]
[quote author=Saxxy link=topic=3603.msg243645#msg243645 date=1335303160]
Research: I'm joining a microbial evolution lab, my PI was previously worked with the E. coli Long-term Experimental Evolution project, so we do a lot of stuff like "hey, can you evolve a microbe to incorporate unnatural amino acids into it's proteins?" or "can a microbe evolve to be a better evolver?"

I don't have a solid, this-is-exactly-what-i'll-do project yet, but the broad outline is going to be what i'm calling "genomic remixing".  There are a lot of systems out there in nature and in labs that can transfer genes between microbes.  Generally this happens at a pretty low frequency, but there's a species of marine microbes that produce these virus-like particles called "gene transfer agents", which could potentially be used to shift relatively large amounts of DNA from a host strain to a recipient strain.  It depends on how these viral-like particles actually interact with the cell they're "infecting", but it has been shown that these guys can infect species that are like, way the hell not related to them (its kinda like the animal equivalent of a jellyfish transfering genes to a human).

Also, I'll be investigating a couple genomic systems to select for microbes that 1) lose large chunks of their genomes (whole or multiple genes at a time), or 2) duplicate chunks of their genome.

And whatever else I have time for/money/energy for, I guess.

Funding: Yeah, it depends on how much funding my PI gets, but I'm paid to be in the lab doing experiments, writing papers, etc.  We're required to do at least one semester of TAing, and it can be more depending on how our funds are.  But yeah, I'm making that sweet sweet grad school cash the pay and stress suck, but I'm doing something I love and to a large extent i'm my own boss aside from what the actual boss-man wants.  Tradeoffs, yo

let's see how happy i am next year, when I'm taking my prelims!

(04-24-2012, 10:53 PM)versus link Wrote: [quote author=Saxxy link=topic=3603.msg243710#msg243710 date=1335325612]
[quote author=versus link=topic=3603.msg243685#msg243685 date=1335320548]
[quote author=Didzo link=topic=3603.msg243678#msg243678 date=1335313773]
[quote author=Saxxy link=topic=3603.msg243645#msg243645 date=1335303160]
Research: I'm joining a microbial evolution lab, my PI was previously worked with the E. coli Long-term Experimental Evolution project, so we do a lot of stuff like "hey, can you evolve a microbe to incorporate unnatural amino acids into it's proteins?" or "can a microbe evolve to be a better evolver?"

I don't have a solid, this-is-exactly-what-i'll-do project yet, but the broad outline is going to be what i'm calling "genomic remixing".  There are a lot of systems out there in nature and in labs that can transfer genes between microbes.  Generally this happens at a pretty low frequency, but there's a species of marine microbes that produce these virus-like particles called "gene transfer agents", which could potentially be used to shift relatively large amounts of DNA from a host strain to a recipient strain.  It depends on how these viral-like particles actually interact with the cell they're "infecting", but it has been shown that these guys can infect species that are like, way the hell not related to them (its kinda like the animal equivalent of a jellyfish transfering genes to a human).

Also, I'll be investigating a couple genomic systems to select for microbes that 1) lose large chunks of their genomes (whole or multiple genes at a time), or 2) duplicate chunks of their genome.

And whatever else I have time for/money/energy for, I guess.

Funding: Yeah, it depends on how much funding my PI gets, but I'm paid to be in the lab doing experiments, writing papers, etc.  We're required to do at least one semester of TAing, and it can be more depending on how our funds are.  But yeah, I'm making that sweet sweet grad school cash the pay and stress suck, but I'm doing something I love and to a large extent i'm my own boss aside from what the actual boss-man wants.  Tradeoffs, yo

let's see how happy i am next year, when I'm taking my prelims!

Quote:[quote author=Saxxy link=topic=3603.msg243713#msg243713 date=1335327070]
yukkii <3 <3

(04-24-2012, 11:19 PM)Caffeine link Wrote:

Quote:[quote author=Saxxy link=topic=3603.msg243713#msg243713 date=1335327070]
yukkii <3 <3

that's an anime thing right

(04-24-2012, 11:19 PM)Caffeine link Wrote:

Quote:[quote author=Saxxy link=topic=3603.msg243713#msg243713 date=1335327070]
yukkii <3 <3

that's an anime thing right

(04-25-2012, 06:46 PM)Surf314 link Wrote: Got a job  ;D